Bactericidal effect of TiO2 photocatalyst on selected food-borne pathogenic bacteria

Titanium dioxide (TiO(2)) photocatalysts have attracted great attention as a material for photocatalytic sterilization in the food and environmental industry. This research aimed to design a new photobioreactor and its application to sterilize selected food borne pathogenic bacteria, Salmonella choleraesuis subsp., Vibrio parahaemolyticus, and Listeria monocytogenes. The photocatalytic reaction was carried out with various TiO(2) concentrations and Ultraviolet (UV) illumination time. A feasible synergistic effect was found that the bactericidal effect of TiO(2) on all bacterial suspension after UV light irradiation was much higher than that of without TiO(2). As the concentration of TiO(2) increased to 1.0 mg/ml, bactericidal effect increased. However, the bactericidal effect was rapidly abbreviated at TiO(2) concentration higher than 1.25 mg/ml to all selected bacteria. UV illumination time affected drastically the viability of all bacteria with different death rate. Similar trends were obtained from S. choleraesuis subsp. and V. parahaemolyticus that their complete killing was achieved after 3 h of illumination. However, L. monocytogenes was more resistant and its death ratio was about 87% at that time.     

Degradation characteristics of toluene,benzene, ethylbenzene,and xylene by Stenotrophomonas maltophilia T3-c

Stenotrophomonas maltophilia T3-c, isolated from a biofilter for the removal of benzene, toluene, ethylbenzene, and xylene (BTEX), could grow in a mineral salt medium containing toluene, benzene, or ethylbenzene as the sole source of carbon. The effect of environmental factors such as initial toluene mass, medium pH, and temperature on the degradation rate of toluene was investigated. The cosubstrate interactions in the BTEX mixture by the isolate were also studied. Within the range of initial toluene mass (from 23 to 70 pmol), an increased substrate concentration increased the specific degradation of toluene by S. maltophilia T3-c. The toluene degradation activity of S. maltophilia T3-c could be maintained at a broad pH range from 5 to 8. The rates at 20 and 40 degrees C were 43 and 83%, respectively, of the rate at 30 degrees C. The specific degradation rates of toluene, benzene, and ethylbenzene by strain T3-c were 2.38, 4.25, and 2.06 micromol/g-DCW/hr. While xylene could not be utilized as a growth substrate by S. maltophilia T3-c, the presence of toluene resulted in the cometabolic degradation of xylene. The specific degradation rate of toluene was increased by the presence of benzene, ethylbenzene, or xylene in binary mixtures. The presence of toluene or xylene in binary mixtures with benzene increased the specific degradation rate of benzene. The presence of ethylbenzene in binary mixtures with benzene inhibited benzene degradation. The presence of more than three kinds of substrates inhibited the specific degradation rate of benzene. All BTEX mixtures, except tri-mixtures of benzene, ethylbenzene, and xylene or mixtures of all four substrates, had little effect on the degradation of ethylbenzene by S. maltophilia T3-c. The utilization preference of the substrates by S. maltophilia T3-c was as follows: ethylbenzene was degraded fastest, followed by toluene and benzene. However, the specific degradation rates of substrates, in order, were benzene, toluene, and ethylbenzene.     

Digested sewage sludge solidification by converter slag for landfill cover

A new technology for solidification of digested sewage sludge referred to as converter slag solidification (CSS) has been developed using converter slag as the solidifying agent and quick lime as the solidifying aid. The CSS technology was investigated by analyzing the physicochemical properties of solidified sludge and determining its microstructural characteristics. The feasibility of using solidified sludge as a landfill cover material was considered in the context of the economical recycling of waste. Sludge solidified using the CSS technology exhibited geotechnical properties that are appropriate for replacing currently used cover soil. Microscopic analyses using XRD, SEM and EDS revealed that the main hydrated product of solidification was CSH (CaO . SiO2 . nH2O), which may play an important role in the effective setting process. Negligible leaching of heavy metals from the solidified sludge was observed. The solidification process of the hydrated sludge, slag and quicklime eliminated the coliform bacteria. Recycled sewage sludge solidified using CCS technology could be used as an effective landfill cover.     

Modeling the buoyant flow of heated water discharged from surface and submerged side outfalls in shallow and deep water with a cross flow

In this study, a three-dimensional model was used to numerically study the buoyant flow, along with its mixing characteristics, of heated water discharged from the surface and submerged side outfalls in shallow and deep water with a cross flow. Hydraulic experimental data were used to evaluate the applicability of the model. The simulation results agree well with the experimental results, particularly, the jet trajectories, the dimensions of the recirculating zone, and the distribution of the dimensionless excess temperature. The level of accuracy of the simulation results of the present study is nearly identical to that of the results conducted by McGuirk and Rodi (1978). If the heated water is discharged into shallow water where the momentum flux ratio and the discharge densimetric Froude number are high, the submerged discharge method is better than the surface discharge method in terms of the scale of the recirculating zone and the minimum dilution. In deep water, where the momentum flux ratio and discharge densimetric Froude number are low, however, the submerged discharge method had few advantages. In shallow water, the discharge jet is deflected by the ambient cross flow, while forcing the ambient flow to bend towards the far bank for the full depth. For a submerged discharge in shallow water, the recirculating zone is the largest in the lowest layer but becomes smaller in the upper layer. As the water depth increases, the ambient flow goes over the jet and diminishes the blocking effect, thereby decreasing the bending of the jet.     

Control targets for the management of biological systems

Attention is focused on biological systems which are describable in terms of ordinary differential equations subject to human control inputs. The concept of an isochronal system is introduced in order to include systems for which the differential equations are valid only over regularly reoccurring time intervals.It is assumed that the control inputs are to be chosen so that an integral cost function of the state of the system, the control used, current time, and the time interval of the control program is minimized. Problems associated with minimizing this cost function over an infinitely long time interval is then considered. Difficulties inherent with minimizing a cost integral on an infinite time interval are shown to be avoided by minimizing an average of the cost function over an unknown but periodic time interval. Under proper circumstance, the optimal control program for the average cost function is either identical to or a good approximation to the optimal control program for the original cost function over an infinitely long time interval.Necessary conditions are obtained for minimizing an average cost function over an unspecified time interval subject to the system equations. For a given problem the necessary conditions will yield but a single system trajectory in the state space. For management purposes this trajectory may be thought of as a target to which the system should be driven and maintained.A number of examples illustrate the use of the necessary conditions to obtain control targets. Certain problems associated with the stability of the target solutions are illustrated with the examples.     

The continuous monitoring of field water samples with a novel multi-channel two-stage mini-bioreactor system

Toxicity monitoring of field water samples was performed using a novel multi-channel two-stage mini-bioreactor system and genetically engineered bioluminescent bacteria for the continuous monitoring and classification of the toxicity present in the samples. The toxicity of various samples spiked with known endocrine disrupting chemicals and phenol was also investigated for system characterization. The field samples used in this study were obtained from two different sites on a monthly basis--from a drinking water treatment plant, referred to as site N, and from a stream near a dam which is currently being constructed, referred to as site T. These samples were either pumped or injected into the second mini-bioreactors to initiate the toxicity test. Most of the samples did not show any specific toxicity. However, one sample showed to have, based upon the detection results, and was deemed toxic. The samples spiked with phenol showed possible responses in the DPD2540 and TV1061 channels, indicating the occurrence of both membrane and protein damage due to phenol. In the tests using an endocrine disrupting chemical, bisphenol A, DNA damage was detected in the DPD2794 channel with a concentration of 2 ppm. Finally, a simple but novel early warning protocol that can be used in a drinking water reservoir and a suspected place where effluents of toxic materials enter the water sourse was suggested with a schematic diagram. In conclusion, this system showed good feasibility for use as a toxicity monitoring system in the field and as an early warning system, indicating if effluents are toxic.     

A multilevel study of group‐focused and individual‐focused transformational leadership,social exchange relationships,and performance in teams

Using matched reports from 73 team leaders and 359 of their members across 23 companies in Korea, we examined a multilevel model where group‐ and individual‐focused transformational leadership and their influence processes operate at the team and dyadic levels independently and interactively to be associated with team and member performance. Results indicated that group‐focused transformational leadership was positively associated with team performance through team member exchange (TMX), whereas individual‐focused transformational leadership positively related to team members' in‐role and extra‐role performance through leader–member exchange (LMX). TMX not only positively mediated the relationships between group‐focused transformational leadership and member performance after controlling for LMX but also positively moderated LMX–performance relationships. Moreover, the indirect effect of individual‐focused transformational leadership through LMX on member performance was contingent upon the level of TMX. Theoretical and applied implications are discussed. Copyright © 2015 John Wiley & Sons, Ltd.     

Degradation of hexane and other recalcitrant hydrocarbons by a novel isolate, <Emphasis Type="Italic">Rhodococcus</Emphasis> sp. EH831

Background, aim, and scope  

Hexane, a representative VOC, is used as a solvent for extraction and as an ingredient in gasoline. The degradation of hexane by bacteria is relatively slow due to its low solubility. Moreover, the biodegradation pathway of hexane under aerobic conditions remains to be investigated; therefore, a study relating to aerobic biodegradation mechanisms is required. Consequently, in this study, an effective hexane degrader was isolated and the biodegradation pathway examined for the first time. In addition, the degradation characteristics of a variety of recalcitrant hydrocarbons were qualitatively and quantitatively investigated using the isolate.     

Assessment of airborne environmental bacteria and related factors in 25 underground railway stations in Seoul,Korea

This study assessed bacterial concentrations in indoor air at 25 underground railway stations in Seoul, Korea, and investigated various related factors including the presence of platform screen doors (PSD), depth of the station, year of construction, temperature, relative humidity, and number of passengers. A total of 72 aerosol samples were collected from all the stations. Concentrations of total airborne bacteria (TAB) ranged from not detected (ND) to 4997 CFU m^?3, with an overall geometric mean (GM) of 191 CFU m^?3. Airborne bacteria were detected at 23 stations (92%) and Gram-negative bacteria (GNB) were detected at two stations (8%). TAB concentrations of four stations (16%) exceeded 800 CFU m^?3, the Korea indoor bio-aerosol guideline. The results of the study showed that TAB concentrations at the stations without PSD showed higher TAB concentrations than those with PSD, though not at statistically significant levels. TAB concentrations of deeper stations revealed significantly higher levels than those of shallower stations. Based on this study, it is recommended that mitigation measures be applied to improve the indoor air quality (IAQ) of underground railway stations in Seoul, with focused attention on deeper stations.     

An evaluation of indoor and outdoor biological particulate matter

The incidences of allergies, allergic diseases and asthma are increasing world wide. Global climate change is likely to impact plants and animals, as well as microorganisms. The World Health Organization, U.S. Environmental Protection Agency, U.S. Department of Agriculture, U.S. Department of Health and Human Services, and the Intergovernmental Panel on Climate Change cite increased allergic reactions due to climate change as a growing concern. Monitoring of indoor and ambient particulate matter (PM) and the characterization of the content for biological aerosol concentrations has not been extensively performed. Samples from urban and rural North Carolina (NC), and Denver (CO), were collected and analyzed as the goal of this research. A study of PM₁₀ (<10 μm in aerodynamic diameter) and PM_2.5 (<2.5 μm in aerodynamic diameter) fractions of ambient bioaerosols was undertaken for a six month period to evaluate the potential for long-term concentrations. These airborne bioaerosols can induce irritational, allergic, infectious, and chemical responses in exposed individuals. Three separate sites were monitored, samples were collected and analyzed for mass and biological content (endotoxins, (1,3)-β-d-glucan and protein). Concentrations of these bioaerosols were reported as a function of PM size fraction, mass and volume of air sampled. The results indicated that higher concentrations of biologicals were present in PM₁₀ than were present in PM_2.5, except when near-roadway conditions existed. This study provides the characterization of ambient bioaerosol concentrations in a variety of areas and conditions.